Isothermal Titration Calorimetry
Complex binding models, global fitting & Kinetics
Upon download, all users will get a FREE 30 days trial. Software Updates and News 12. Binding Independent Sites Isothermal Titration Calorimetry Isotherm. Isothermal Titration Calorimetry of Protein—Protein Interactions Michael M. Molecule concentration including bound and free frac-tions, K a is the binding constant, and L is the free. Titrant is added to the sample cell containing the mac-romolecule. In a separate (control) experiment titrant.
KinITC
Study the isothermal GuHCl induced unfolding by isothermal titration calorimetry, ITC, and circular dichroism, CD, at 25 C. The use of isothermal calorimetry to characterise and quantify the unfolding behaviour of proteins in the presence of denaturants has not gained much attention and use. Pfeil and Privalov used isothermal. Isothermal titration calorimetry (ITC) is a useful tool for understanding the complete thermodynamic picture of a binding reaction. Experimental Thermodynamics, Volume 1: Calorimetry of Non-Reacting Systems covers the heat capacity determinations for chemical substances in the solid, liquid, solution, and vapor states, at temperatures ranging. Isothermal titration calorimetry (ITC) is a physical technique used to determine the thermodynamic parameters of interactions in solution. It is most often used to study the binding of small molecules (such as medicinal compounds) to larger macromolecules (proteins, DNA etc.). It consists of two cells which are enclosed in an adiabatic jacket. The compounds to be studied are placed in the.
An original tool to get the full thermodynamic (KD, ΔH) and kinetic (kon, koff) profiles of the formation of 1:1 and 1:n ligand-receptor complexes. The information is obtained directly from standard ITC experiments, in an experimental environment “label-free” and “in solution”.
Model Builder
This tool allows users to create original stoichiometric binding models through an easy-to-use and clean interface. These models can be applied to the analysis of a plethora of binding interactions of higher complexity, like those involving binding heterogeneity, auto-association induced by binding, cooperative effects or conformational changes, to mention few.
Global Analysis
Upload several isotherms to the same fitting project and perform a global fitting using the same (or different) binding model for each isotherm; define global parameters “linked” across two or more isotherms or even relate parameters using mathematical equations. This tool is incredibly useful to assess data quality and reproducibility, and indispensable for the robust analysis of complex interactions.
Simulate isotherms
Find the best experimental condition to perform your experiments, even with complex interactions.
Check Local minima
Check how reliable your results are and detect over-parametrization of the analyses.
Get advantage of supercomputing
Our cloud version of the software uses supercomputing system to offer you a faster and more reliable analysis.
Obtain Scientific reports
Isothermal Titration Calorimetry Software Mac Free Download Windows 7
Get all you data, figures, tables and tables ready for publications and presentations.